Transfection:Article Title: Unmasking subtype-dependent susceptibility to C-type inactivation in mammalian Kv1 channels.
Article Snippet: Shaker potassium channels have been an essential model for studying inactivation of ion channels and shaped our earliest understanding of N-type vs. C-type mechanisms.. In early work describing C-type inactivation, López-Barneo and colleagues systematically characterized numerous mutations of Shaker residue T449, demonstrating that this position was a key determinant of C-type inactivation rate.. In most of the closely related mammalian Kv1 channels, however, a persistent enigma has been that residue identity at this position has relatively modest effects on the rate of inactivation in response to long depolarizations.
Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat#87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2
Article Title: Exonic splicing code and coordination of divalent metals in proteins.
Article Snippet: .. Transfections were carried out in 12- or 4-well plates using 150 ng of reporter DNA and jetPRIME Polyplus) according to the manufacturer’s recommendations. he cells were lysed 24 h later for RNA extraction. .. Total NA was isolated using TRI Reagent (Molecular Research enter) according to the manufacturer’s protocol and used or the first-strand cDNA synthesis with the Moloney murine eukaemia virus reverse transcriptase (Promega) and oligo (T) primers.
Plasmid Preparation:
Immunocytochemistry:
Construct:
Virus:Article Title: Electrochemical coupling at the plasma membrane by mouse voltage-sensitive phosphatase requires association with basigin.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Antibody (B-2) Santa Cruz Biotechnology Cat#sc-9996; RRID: AB_627695 ANTI-FLAG® M2 antibody Sigma-Aldrich Cat#F1804; RRID: AB_262044 Myc-Tag (71D10) Rabbit mAb Cell Signaling Technology Cat#91856; RRID: AB_3073821 Anti-Human HA Monoclonal Antibody Sigma-Aldrich Cat#H3663; RRID: AB_262051 Lex A (2-12) Santa Cruz Biotechnology Cat# sc-7544; RRID AB_627883 IRDye 800CW Goat anti-Mouse IgG LI-COR Biosciences Cat#925-32210; RRID: AB_2687825 IRDye 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 RDye 800CW Goat anti-Rat IgG LI-COR Biosciences Cat#926-32219, RRID: AB_1850025 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat#A-11029; RRID: AB_2534088 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat#A-11032; RRID: AB_2534091 Mouse EMMPRIN/CD147 Antibody R and D Systems Cat#AF772; RRID: AB_355588 Donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat# sc-2020; RRID: AB_631728 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Hygromycin B Thermo Fisher Scientific Cat#10687010 GelRed® Nucleic Acid Stain Merck Cat#SCT123 Protease-Inhibitor-Mix G SERVA Cat#39101 Triton® X 100 ROTH Cat#3051.3 Poly(ethyleneglycol) Sigma-Aldrich Cat#81242 Minimal SD Base Takara Cat#630411 DO Supplement –Leu/–Trp Takara Cat#630417 DO Supplement –His/–Leu/–Trp Takara Cat#630419 DO Supplement –Ade/–His/–Leu/–Trp Takara Cat#630428 YPD Broth ROTH Cat#X970 Lithium acetate dihydrate Sigma-Aldrich Cat#L4158 Deoxyribonucleic acid from herring sperm Sigma-Aldrich Cat#D3159 jetPEI® Polyplus Cat#101000053 jetPRIME® Polyplus Cat101000046 Critical commercial assays Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#M8823 Y2H Membrane Protein Kit MoBiTec GmbH Cat#P01001DS PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 Phusion High-Fidelity PCR Master Mix with HF Buffer Thermo ScientificTM Cat#F531L SuperSignalTM ELISA Femto Substrate Thermo ScientificTM Cat#37075 Deposited data Source data https://doi.org/10.6084/ m9.figshare.29665130 N/A (Continued on next page) Cell Reports 44, 116200, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK-293, Human Cytion Cat#CLS-300192 HeLa, Human Biozol Cat#ADD-C0008001 CHO-K1, hamster Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat#ACC110 ROMK2-Flp-In 293 Cells Renigunta et al.47 N/A Experimental models: Organisms/strains Strain NMY51: [MATa his3Δ200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ ade2::(lexAop)8-ADE2 GAL4] MoBiTec GmbH N/A Oligonucleotides mVSP pBT3C for: 5’-aat agg cca tta cgg cca tgt atg gag aa-3’ This paper N/A mVSP pBT3C rev: 5’-att tgg ccg agg cgg ccc tgt tct cac caa a-3’ This paper N/A mBasigin-pPR3C-for: 5’-att ggc cat tac ggc ccg atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin-pPR3C-rev: 5’-aat ggc cga ggc ggc cga ggt ggc gtt cct ctg-3’ This paper N/A mVSP-PEGFPC-for: 5’-gat ctc gag cta tgt atg gag aaa aga ag-3’ This paper N/A mVSP-PEGFPC-rev: 5’-ggt gga tcc tca ctg cag cta gtt ctc acc aaa-3’ This paper N/A mBasigin pRFPN for: 5’-gat ctc gag atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin pRFPN rev: 5’-ggt gga tcc cgg gtg gcg ttc ct-3’ This paper N/A mVSP exHA for: 5’-gca ctt tat gac aaa cca ggc ggg tac cca tac gac gtc cca gat tac gct ggc ggg cct cac ttt ctc aga g-3’ This paper N/A mVSP exHA rev: 5’-ctc tga gaa agt gag gcc cgc cag cgt aat ctg gga cgt cgt atg ggt acc cgc ctg gtt tgt cat aaa gtg c-3’ This paper N/A Recombinant DNA mVSP-pEGFPC This paper Genbank: NM_199257.2 mVSP-pRFP This paper Genbank: NM_199257.2 mVSP-pBT3N This paper Genbank: NM_199257.2 mVSP-3xflag-pCDNA3.1 This paper Genbank: NM_199257.2 mVSP-Ex-HA-pEGFP This paper Genbank: NM_199257.2 m/ci-VSP-pEGFP This paper N/A ci/m-VSP-pEGFP This paper N/A mBSG-pPR3C This paper Genbank: NM_001077184.1 mBSG-pRFP This paper Genbank: NM_001077184.1 mBSG-myc-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-3xflag-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-ERret-pRFP This paper N/A mEMB-pRFP This paper Genbank: NM_010330.4 mEMB -ERret-pRFP This paper N/A mNPTN-pRFP This paper Genbank: NM_009145.2 (Continued on next page) 18 Cell Reports 44, 116200, September 23, 2025
Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat#87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2
Subcloning:Article Title: Electrochemical coupling at the plasma membrane by mouse voltage-sensitive phosphatase requires association with basigin.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Antibody (B-2) Santa Cruz Biotechnology Cat#sc-9996; RRID: AB_627695 ANTI-FLAG® M2 antibody Sigma-Aldrich Cat#F1804; RRID: AB_262044 Myc-Tag (71D10) Rabbit mAb Cell Signaling Technology Cat#91856; RRID: AB_3073821 Anti-Human HA Monoclonal Antibody Sigma-Aldrich Cat#H3663; RRID: AB_262051 Lex A (2-12) Santa Cruz Biotechnology Cat# sc-7544; RRID AB_627883 IRDye 800CW Goat anti-Mouse IgG LI-COR Biosciences Cat#925-32210; RRID: AB_2687825 IRDye 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 RDye 800CW Goat anti-Rat IgG LI-COR Biosciences Cat#926-32219, RRID: AB_1850025 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat#A-11029; RRID: AB_2534088 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat#A-11032; RRID: AB_2534091 Mouse EMMPRIN/CD147 Antibody R and D Systems Cat#AF772; RRID: AB_355588 Donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat# sc-2020; RRID: AB_631728 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Hygromycin B Thermo Fisher Scientific Cat#10687010 GelRed® Nucleic Acid Stain Merck Cat#SCT123 Protease-Inhibitor-Mix G SERVA Cat#39101 Triton® X 100 ROTH Cat#3051.3 Poly(ethyleneglycol) Sigma-Aldrich Cat#81242 Minimal SD Base Takara Cat#630411 DO Supplement –Leu/–Trp Takara Cat#630417 DO Supplement –His/–Leu/–Trp Takara Cat#630419 DO Supplement –Ade/–His/–Leu/–Trp Takara Cat#630428 YPD Broth ROTH Cat#X970 Lithium acetate dihydrate Sigma-Aldrich Cat#L4158 Deoxyribonucleic acid from herring sperm Sigma-Aldrich Cat#D3159 jetPEI® Polyplus Cat#101000053 jetPRIME® Polyplus Cat101000046 Critical commercial assays Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#M8823 Y2H Membrane Protein Kit MoBiTec GmbH Cat#P01001DS PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 Phusion High-Fidelity PCR Master Mix with HF Buffer Thermo ScientificTM Cat#F531L SuperSignalTM ELISA Femto Substrate Thermo ScientificTM Cat#37075 Deposited data Source data https://doi.org/10.6084/ m9.figshare.29665130 N/A (Continued on next page) Cell Reports 44, 116200, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK-293, Human Cytion Cat#CLS-300192 HeLa, Human Biozol Cat#ADD-C0008001 CHO-K1, hamster Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat#ACC110 ROMK2-Flp-In 293 Cells Renigunta et al.47 N/A Experimental models: Organisms/strains Strain NMY51: [MATa his3Δ200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ ade2::(lexAop)8-ADE2 GAL4] MoBiTec GmbH N/A Oligonucleotides mVSP pBT3C for: 5’-aat agg cca tta cgg cca tgt atg gag aa-3’ This paper N/A mVSP pBT3C rev: 5’-att tgg ccg agg cgg ccc tgt tct cac caa a-3’ This paper N/A mBasigin-pPR3C-for: 5’-att ggc cat tac ggc ccg atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin-pPR3C-rev: 5’-aat ggc cga ggc ggc cga ggt ggc gtt cct ctg-3’ This paper N/A mVSP-PEGFPC-for: 5’-gat ctc gag cta tgt atg gag aaa aga ag-3’ This paper N/A mVSP-PEGFPC-rev: 5’-ggt gga tcc tca ctg cag cta gtt ctc acc aaa-3’ This paper N/A mBasigin pRFPN for: 5’-gat ctc gag atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin pRFPN rev: 5’-ggt gga tcc cgg gtg gcg ttc ct-3’ This paper N/A mVSP exHA for: 5’-gca ctt tat gac aaa cca ggc ggg tac cca tac gac gtc cca gat tac gct ggc ggg cct cac ttt ctc aga g-3’ This paper N/A mVSP exHA rev: 5’-ctc tga gaa agt gag gcc cgc cag cgt aat ctg gga cgt cgt atg ggt acc cgc ctg gtt tgt cat aaa gtg c-3’ This paper N/A Recombinant DNA mVSP-pEGFPC This paper Genbank: NM_199257.2 mVSP-pRFP This paper Genbank: NM_199257.2 mVSP-pBT3N This paper Genbank: NM_199257.2 mVSP-3xflag-pCDNA3.1 This paper Genbank: NM_199257.2 mVSP-Ex-HA-pEGFP This paper Genbank: NM_199257.2 m/ci-VSP-pEGFP This paper N/A ci/m-VSP-pEGFP This paper N/A mBSG-pPR3C This paper Genbank: NM_001077184.1 mBSG-pRFP This paper Genbank: NM_001077184.1 mBSG-myc-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-3xflag-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-ERret-pRFP This paper N/A mEMB-pRFP This paper Genbank: NM_010330.4 mEMB -ERret-pRFP This paper N/A mNPTN-pRFP This paper Genbank: NM_009145.2 (Continued on next page) 18 Cell Reports 44, 116200, September 23, 2025
Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat#87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2
Recombinant:Article Title: Electrochemical coupling at the plasma membrane by mouse voltage-sensitive phosphatase requires association with basigin.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Antibody (B-2) Santa Cruz Biotechnology Cat#sc-9996; RRID: AB_627695 ANTI-FLAG® M2 antibody Sigma-Aldrich Cat#F1804; RRID: AB_262044 Myc-Tag (71D10) Rabbit mAb Cell Signaling Technology Cat#91856; RRID: AB_3073821 Anti-Human HA Monoclonal Antibody Sigma-Aldrich Cat#H3663; RRID: AB_262051 Lex A (2-12) Santa Cruz Biotechnology Cat# sc-7544; RRID AB_627883 IRDye 800CW Goat anti-Mouse IgG LI-COR Biosciences Cat#925-32210; RRID: AB_2687825 IRDye 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 RDye 800CW Goat anti-Rat IgG LI-COR Biosciences Cat#926-32219, RRID: AB_1850025 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat#A-11029; RRID: AB_2534088 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat#A-11032; RRID: AB_2534091 Mouse EMMPRIN/CD147 Antibody R and D Systems Cat#AF772; RRID: AB_355588 Donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat# sc-2020; RRID: AB_631728 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Hygromycin B Thermo Fisher Scientific Cat#10687010 GelRed® Nucleic Acid Stain Merck Cat#SCT123 Protease-Inhibitor-Mix G SERVA Cat#39101 Triton® X 100 ROTH Cat#3051.3 Poly(ethyleneglycol) Sigma-Aldrich Cat#81242 Minimal SD Base Takara Cat#630411 DO Supplement –Leu/–Trp Takara Cat#630417 DO Supplement –His/–Leu/–Trp Takara Cat#630419 DO Supplement –Ade/–His/–Leu/–Trp Takara Cat#630428 YPD Broth ROTH Cat#X970 Lithium acetate dihydrate Sigma-Aldrich Cat#L4158 Deoxyribonucleic acid from herring sperm Sigma-Aldrich Cat#D3159 jetPEI® Polyplus Cat#101000053 jetPRIME® Polyplus Cat101000046 Critical commercial assays Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#M8823 Y2H Membrane Protein Kit MoBiTec GmbH Cat#P01001DS PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 Phusion High-Fidelity PCR Master Mix with HF Buffer Thermo ScientificTM Cat#F531L SuperSignalTM ELISA Femto Substrate Thermo ScientificTM Cat#37075 Deposited data Source data https://doi.org/10.6084/ m9.figshare.29665130 N/A (Continued on next page) Cell Reports 44, 116200, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK-293, Human Cytion Cat#CLS-300192 HeLa, Human Biozol Cat#ADD-C0008001 CHO-K1, hamster Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat#ACC110 ROMK2-Flp-In 293 Cells Renigunta et al.47 N/A Experimental models: Organisms/strains Strain NMY51: [MATa his3Δ200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ ade2::(lexAop)8-ADE2 GAL4] MoBiTec GmbH N/A Oligonucleotides mVSP pBT3C for: 5’-aat agg cca tta cgg cca tgt atg gag aa-3’ This paper N/A mVSP pBT3C rev: 5’-att tgg ccg agg cgg ccc tgt tct cac caa a-3’ This paper N/A mBasigin-pPR3C-for: 5’-att ggc cat tac ggc ccg atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin-pPR3C-rev: 5’-aat ggc cga ggc ggc cga ggt ggc gtt cct ctg-3’ This paper N/A mVSP-PEGFPC-for: 5’-gat ctc gag cta tgt atg gag aaa aga ag-3’ This paper N/A mVSP-PEGFPC-rev: 5’-ggt gga tcc tca ctg cag cta gtt ctc acc aaa-3’ This paper N/A mBasigin pRFPN for: 5’-gat ctc gag atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin pRFPN rev: 5’-ggt gga tcc cgg gtg gcg ttc ct-3’ This paper N/A mVSP exHA for: 5’-gca ctt tat gac aaa cca ggc ggg tac cca tac gac gtc cca gat tac gct ggc ggg cct cac ttt ctc aga g-3’ This paper N/A mVSP exHA rev: 5’-ctc tga gaa agt gag gcc cgc cag cgt aat ctg gga cgt cgt atg ggt acc cgc ctg gtt tgt cat aaa gtg c-3’ This paper N/A Recombinant DNA mVSP-pEGFPC This paper Genbank: NM_199257.2 mVSP-pRFP This paper Genbank: NM_199257.2 mVSP-pBT3N This paper Genbank: NM_199257.2 mVSP-3xflag-pCDNA3.1 This paper Genbank: NM_199257.2 mVSP-Ex-HA-pEGFP This paper Genbank: NM_199257.2 m/ci-VSP-pEGFP This paper N/A ci/m-VSP-pEGFP This paper N/A mBSG-pPR3C This paper Genbank: NM_001077184.1 mBSG-pRFP This paper Genbank: NM_001077184.1 mBSG-myc-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-3xflag-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-ERret-pRFP This paper N/A mEMB-pRFP This paper Genbank: NM_010330.4 mEMB -ERret-pRFP This paper N/A mNPTN-pRFP This paper Genbank: NM_009145.2 (Continued on next page) 18 Cell Reports 44, 116200, September 23, 2025
Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat#87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2
Staining:Article Title: Electrochemical coupling at the plasma membrane by mouse voltage-sensitive phosphatase requires association with basigin.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Antibody (B-2) Santa Cruz Biotechnology Cat#sc-9996; RRID: AB_627695 ANTI-FLAG® M2 antibody Sigma-Aldrich Cat#F1804; RRID: AB_262044 Myc-Tag (71D10) Rabbit mAb Cell Signaling Technology Cat#91856; RRID: AB_3073821 Anti-Human HA Monoclonal Antibody Sigma-Aldrich Cat#H3663; RRID: AB_262051 Lex A (2-12) Santa Cruz Biotechnology Cat# sc-7544; RRID AB_627883 IRDye 800CW Goat anti-Mouse IgG LI-COR Biosciences Cat#925-32210; RRID: AB_2687825 IRDye 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 RDye 800CW Goat anti-Rat IgG LI-COR Biosciences Cat#926-32219, RRID: AB_1850025 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat#A-11029; RRID: AB_2534088 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat#A-11032; RRID: AB_2534091 Mouse EMMPRIN/CD147 Antibody R and D Systems Cat#AF772; RRID: AB_355588 Donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat# sc-2020; RRID: AB_631728 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Hygromycin B Thermo Fisher Scientific Cat#10687010 GelRed® Nucleic Acid Stain Merck Cat#SCT123 Protease-Inhibitor-Mix G SERVA Cat#39101 Triton® X 100 ROTH Cat#3051.3 Poly(ethyleneglycol) Sigma-Aldrich Cat#81242 Minimal SD Base Takara Cat#630411 DO Supplement –Leu/–Trp Takara Cat#630417 DO Supplement –His/–Leu/–Trp Takara Cat#630419 DO Supplement –Ade/–His/–Leu/–Trp Takara Cat#630428 YPD Broth ROTH Cat#X970 Lithium acetate dihydrate Sigma-Aldrich Cat#L4158 Deoxyribonucleic acid from herring sperm Sigma-Aldrich Cat#D3159 jetPEI® Polyplus Cat#101000053 jetPRIME® Polyplus Cat101000046 Critical commercial assays Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#M8823 Y2H Membrane Protein Kit MoBiTec GmbH Cat#P01001DS PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 Phusion High-Fidelity PCR Master Mix with HF Buffer Thermo ScientificTM Cat#F531L SuperSignalTM ELISA Femto Substrate Thermo ScientificTM Cat#37075 Deposited data Source data https://doi.org/10.6084/ m9.figshare.29665130 N/A (Continued on next page) Cell Reports 44, 116200, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK-293, Human Cytion Cat#CLS-300192 HeLa, Human Biozol Cat#ADD-C0008001 CHO-K1, hamster Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat#ACC110 ROMK2-Flp-In 293 Cells Renigunta et al.47 N/A Experimental models: Organisms/strains Strain NMY51: [MATa his3Δ200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ ade2::(lexAop)8-ADE2 GAL4] MoBiTec GmbH N/A Oligonucleotides mVSP pBT3C for: 5’-aat agg cca tta cgg cca tgt atg gag aa-3’ This paper N/A mVSP pBT3C rev: 5’-att tgg ccg agg cgg ccc tgt tct cac caa a-3’ This paper N/A mBasigin-pPR3C-for: 5’-att ggc cat tac ggc ccg atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin-pPR3C-rev: 5’-aat ggc cga ggc ggc cga ggt ggc gtt cct ctg-3’ This paper N/A mVSP-PEGFPC-for: 5’-gat ctc gag cta tgt atg gag aaa aga ag-3’ This paper N/A mVSP-PEGFPC-rev: 5’-ggt gga tcc tca ctg cag cta gtt ctc acc aaa-3’ This paper N/A mBasigin pRFPN for: 5’-gat ctc gag atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin pRFPN rev: 5’-ggt gga tcc cgg gtg gcg ttc ct-3’ This paper N/A mVSP exHA for: 5’-gca ctt tat gac aaa cca ggc ggg tac cca tac gac gtc cca gat tac gct ggc ggg cct cac ttt ctc aga g-3’ This paper N/A mVSP exHA rev: 5’-ctc tga gaa agt gag gcc cgc cag cgt aat ctg gga cgt cgt atg ggt acc cgc ctg gtt tgt cat aaa gtg c-3’ This paper N/A Recombinant DNA mVSP-pEGFPC This paper Genbank: NM_199257.2 mVSP-pRFP This paper Genbank: NM_199257.2 mVSP-pBT3N This paper Genbank: NM_199257.2 mVSP-3xflag-pCDNA3.1 This paper Genbank: NM_199257.2 mVSP-Ex-HA-pEGFP This paper Genbank: NM_199257.2 m/ci-VSP-pEGFP This paper N/A ci/m-VSP-pEGFP This paper N/A mBSG-pPR3C This paper Genbank: NM_001077184.1 mBSG-pRFP This paper Genbank: NM_001077184.1 mBSG-myc-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-3xflag-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-ERret-pRFP This paper N/A mEMB-pRFP This paper Genbank: NM_010330.4 mEMB -ERret-pRFP This paper N/A mNPTN-pRFP This paper Genbank: NM_009145.2 (Continued on next page) 18 Cell Reports 44, 116200, September 23, 2025
Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat#87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2
Magnetic Beads:Article Title: Electrochemical coupling at the plasma membrane by mouse voltage-sensitive phosphatase requires association with basigin.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Antibody (B-2) Santa Cruz Biotechnology Cat#sc-9996; RRID: AB_627695 ANTI-FLAG® M2 antibody Sigma-Aldrich Cat#F1804; RRID: AB_262044 Myc-Tag (71D10) Rabbit mAb Cell Signaling Technology Cat#91856; RRID: AB_3073821 Anti-Human HA Monoclonal Antibody Sigma-Aldrich Cat#H3663; RRID: AB_262051 Lex A (2-12) Santa Cruz Biotechnology Cat# sc-7544; RRID AB_627883 IRDye 800CW Goat anti-Mouse IgG LI-COR Biosciences Cat#925-32210; RRID: AB_2687825 IRDye 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 RDye 800CW Goat anti-Rat IgG LI-COR Biosciences Cat#926-32219, RRID: AB_1850025 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat#A-11029; RRID: AB_2534088 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat#A-11032; RRID: AB_2534091 Mouse EMMPRIN/CD147 Antibody R and D Systems Cat#AF772; RRID: AB_355588 Donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat# sc-2020; RRID: AB_631728 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Hygromycin B Thermo Fisher Scientific Cat#10687010 GelRed® Nucleic Acid Stain Merck Cat#SCT123 Protease-Inhibitor-Mix G SERVA Cat#39101 Triton® X 100 ROTH Cat#3051.3 Poly(ethyleneglycol) Sigma-Aldrich Cat#81242 Minimal SD Base Takara Cat#630411 DO Supplement –Leu/–Trp Takara Cat#630417 DO Supplement –His/–Leu/–Trp Takara Cat#630419 DO Supplement –Ade/–His/–Leu/–Trp Takara Cat#630428 YPD Broth ROTH Cat#X970 Lithium acetate dihydrate Sigma-Aldrich Cat#L4158 Deoxyribonucleic acid from herring sperm Sigma-Aldrich Cat#D3159 jetPEI® Polyplus Cat#101000053 jetPRIME® Polyplus Cat101000046 Critical commercial assays Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#M8823 Y2H Membrane Protein Kit MoBiTec GmbH Cat#P01001DS PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 Phusion High-Fidelity PCR Master Mix with HF Buffer Thermo ScientificTM Cat#F531L SuperSignalTM ELISA Femto Substrate Thermo ScientificTM Cat#37075 Deposited data Source data https://doi.org/10.6084/ m9.figshare.29665130 N/A (Continued on next page) Cell Reports 44, 116200, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK-293, Human Cytion Cat#CLS-300192 HeLa, Human Biozol Cat#ADD-C0008001 CHO-K1, hamster Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat#ACC110 ROMK2-Flp-In 293 Cells Renigunta et al.47 N/A Experimental models: Organisms/strains Strain NMY51: [MATa his3Δ200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ ade2::(lexAop)8-ADE2 GAL4] MoBiTec GmbH N/A Oligonucleotides mVSP pBT3C for: 5’-aat agg cca tta cgg cca tgt atg gag aa-3’ This paper N/A mVSP pBT3C rev: 5’-att tgg ccg agg cgg ccc tgt tct cac caa a-3’ This paper N/A mBasigin-pPR3C-for: 5’-att ggc cat tac ggc ccg atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin-pPR3C-rev: 5’-aat ggc cga ggc ggc cga ggt ggc gtt cct ctg-3’ This paper N/A mVSP-PEGFPC-for: 5’-gat ctc gag cta tgt atg gag aaa aga ag-3’ This paper N/A mVSP-PEGFPC-rev: 5’-ggt gga tcc tca ctg cag cta gtt ctc acc aaa-3’ This paper N/A mBasigin pRFPN for: 5’-gat ctc gag atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin pRFPN rev: 5’-ggt gga tcc cgg gtg gcg ttc ct-3’ This paper N/A mVSP exHA for: 5’-gca ctt tat gac aaa cca ggc ggg tac cca tac gac gtc cca gat tac gct ggc ggg cct cac ttt ctc aga g-3’ This paper N/A mVSP exHA rev: 5’-ctc tga gaa agt gag gcc cgc cag cgt aat ctg gga cgt cgt atg ggt acc cgc ctg gtt tgt cat aaa gtg c-3’ This paper N/A Recombinant DNA mVSP-pEGFPC This paper Genbank: NM_199257.2 mVSP-pRFP This paper Genbank: NM_199257.2 mVSP-pBT3N This paper Genbank: NM_199257.2 mVSP-3xflag-pCDNA3.1 This paper Genbank: NM_199257.2 mVSP-Ex-HA-pEGFP This paper Genbank: NM_199257.2 m/ci-VSP-pEGFP This paper N/A ci/m-VSP-pEGFP This paper N/A mBSG-pPR3C This paper Genbank: NM_001077184.1 mBSG-pRFP This paper Genbank: NM_001077184.1 mBSG-myc-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-3xflag-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-ERret-pRFP This paper N/A mEMB-pRFP This paper Genbank: NM_010330.4 mEMB -ERret-pRFP This paper N/A mNPTN-pRFP This paper Genbank: NM_009145.2 (Continued on next page) 18 Cell Reports 44, 116200, September 23, 2025
Membrane:Article Title: Electrochemical coupling at the plasma membrane by mouse voltage-sensitive phosphatase requires association with basigin.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Antibody (B-2) Santa Cruz Biotechnology Cat#sc-9996; RRID: AB_627695 ANTI-FLAG® M2 antibody Sigma-Aldrich Cat#F1804; RRID: AB_262044 Myc-Tag (71D10) Rabbit mAb Cell Signaling Technology Cat#91856; RRID: AB_3073821 Anti-Human HA Monoclonal Antibody Sigma-Aldrich Cat#H3663; RRID: AB_262051 Lex A (2-12) Santa Cruz Biotechnology Cat# sc-7544; RRID AB_627883 IRDye 800CW Goat anti-Mouse IgG LI-COR Biosciences Cat#925-32210; RRID: AB_2687825 IRDye 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 RDye 800CW Goat anti-Rat IgG LI-COR Biosciences Cat#926-32219, RRID: AB_1850025 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat#A-11029; RRID: AB_2534088 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat#A-11032; RRID: AB_2534091 Mouse EMMPRIN/CD147 Antibody R and D Systems Cat#AF772; RRID: AB_355588 Donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat# sc-2020; RRID: AB_631728 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Hygromycin B Thermo Fisher Scientific Cat#10687010 GelRed® Nucleic Acid Stain Merck Cat#SCT123 Protease-Inhibitor-Mix G SERVA Cat#39101 Triton® X 100 ROTH Cat#3051.3 Poly(ethyleneglycol) Sigma-Aldrich Cat#81242 Minimal SD Base Takara Cat#630411 DO Supplement –Leu/–Trp Takara Cat#630417 DO Supplement –His/–Leu/–Trp Takara Cat#630419 DO Supplement –Ade/–His/–Leu/–Trp Takara Cat#630428 YPD Broth ROTH Cat#X970 Lithium acetate dihydrate Sigma-Aldrich Cat#L4158 Deoxyribonucleic acid from herring sperm Sigma-Aldrich Cat#D3159 jetPEI® Polyplus Cat#101000053 jetPRIME® Polyplus Cat101000046 Critical commercial assays Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#M8823 Y2H Membrane Protein Kit MoBiTec GmbH Cat#P01001DS PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 Phusion High-Fidelity PCR Master Mix with HF Buffer Thermo ScientificTM Cat#F531L SuperSignalTM ELISA Femto Substrate Thermo ScientificTM Cat#37075 Deposited data Source data https://doi.org/10.6084/ m9.figshare.29665130 N/A (Continued on next page) Cell Reports 44, 116200, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK-293, Human Cytion Cat#CLS-300192 HeLa, Human Biozol Cat#ADD-C0008001 CHO-K1, hamster Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat#ACC110 ROMK2-Flp-In 293 Cells Renigunta et al.47 N/A Experimental models: Organisms/strains Strain NMY51: [MATa his3Δ200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ ade2::(lexAop)8-ADE2 GAL4] MoBiTec GmbH N/A Oligonucleotides mVSP pBT3C for: 5’-aat agg cca tta cgg cca tgt atg gag aa-3’ This paper N/A mVSP pBT3C rev: 5’-att tgg ccg agg cgg ccc tgt tct cac caa a-3’ This paper N/A mBasigin-pPR3C-for: 5’-att ggc cat tac ggc ccg atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin-pPR3C-rev: 5’-aat ggc cga ggc ggc cga ggt ggc gtt cct ctg-3’ This paper N/A mVSP-PEGFPC-for: 5’-gat ctc gag cta tgt atg gag aaa aga ag-3’ This paper N/A mVSP-PEGFPC-rev: 5’-ggt gga tcc tca ctg cag cta gtt ctc acc aaa-3’ This paper N/A mBasigin pRFPN for: 5’-gat ctc gag atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin pRFPN rev: 5’-ggt gga tcc cgg gtg gcg ttc ct-3’ This paper N/A mVSP exHA for: 5’-gca ctt tat gac aaa cca ggc ggg tac cca tac gac gtc cca gat tac gct ggc ggg cct cac ttt ctc aga g-3’ This paper N/A mVSP exHA rev: 5’-ctc tga gaa agt gag gcc cgc cag cgt aat ctg gga cgt cgt atg ggt acc cgc ctg gtt tgt cat aaa gtg c-3’ This paper N/A Recombinant DNA mVSP-pEGFPC This paper Genbank: NM_199257.2 mVSP-pRFP This paper Genbank: NM_199257.2 mVSP-pBT3N This paper Genbank: NM_199257.2 mVSP-3xflag-pCDNA3.1 This paper Genbank: NM_199257.2 mVSP-Ex-HA-pEGFP This paper Genbank: NM_199257.2 m/ci-VSP-pEGFP This paper N/A ci/m-VSP-pEGFP This paper N/A mBSG-pPR3C This paper Genbank: NM_001077184.1 mBSG-pRFP This paper Genbank: NM_001077184.1 mBSG-myc-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-3xflag-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-ERret-pRFP This paper N/A mEMB-pRFP This paper Genbank: NM_010330.4 mEMB -ERret-pRFP This paper N/A mNPTN-pRFP This paper Genbank: NM_009145.2 (Continued on next page) 18 Cell Reports 44, 116200, September 23, 2025
Polymerase Chain Reaction:Article Title: Electrochemical coupling at the plasma membrane by mouse voltage-sensitive phosphatase requires association with basigin.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Antibody (B-2) Santa Cruz Biotechnology Cat#sc-9996; RRID: AB_627695 ANTI-FLAG® M2 antibody Sigma-Aldrich Cat#F1804; RRID: AB_262044 Myc-Tag (71D10) Rabbit mAb Cell Signaling Technology Cat#91856; RRID: AB_3073821 Anti-Human HA Monoclonal Antibody Sigma-Aldrich Cat#H3663; RRID: AB_262051 Lex A (2-12) Santa Cruz Biotechnology Cat# sc-7544; RRID AB_627883 IRDye 800CW Goat anti-Mouse IgG LI-COR Biosciences Cat#925-32210; RRID: AB_2687825 IRDye 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 RDye 800CW Goat anti-Rat IgG LI-COR Biosciences Cat#926-32219, RRID: AB_1850025 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat#A-11029; RRID: AB_2534088 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat#A-11032; RRID: AB_2534091 Mouse EMMPRIN/CD147 Antibody R and D Systems Cat#AF772; RRID: AB_355588 Donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat# sc-2020; RRID: AB_631728 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Hygromycin B Thermo Fisher Scientific Cat#10687010 GelRed® Nucleic Acid Stain Merck Cat#SCT123 Protease-Inhibitor-Mix G SERVA Cat#39101 Triton® X 100 ROTH Cat#3051.3 Poly(ethyleneglycol) Sigma-Aldrich Cat#81242 Minimal SD Base Takara Cat#630411 DO Supplement –Leu/–Trp Takara Cat#630417 DO Supplement –His/–Leu/–Trp Takara Cat#630419 DO Supplement –Ade/–His/–Leu/–Trp Takara Cat#630428 YPD Broth ROTH Cat#X970 Lithium acetate dihydrate Sigma-Aldrich Cat#L4158 Deoxyribonucleic acid from herring sperm Sigma-Aldrich Cat#D3159 jetPEI® Polyplus Cat#101000053 jetPRIME® Polyplus Cat101000046 Critical commercial assays Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#M8823 Y2H Membrane Protein Kit MoBiTec GmbH Cat#P01001DS PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 Phusion High-Fidelity PCR Master Mix with HF Buffer Thermo ScientificTM Cat#F531L SuperSignalTM ELISA Femto Substrate Thermo ScientificTM Cat#37075 Deposited data Source data https://doi.org/10.6084/ m9.figshare.29665130 N/A (Continued on next page) Cell Reports 44, 116200, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK-293, Human Cytion Cat#CLS-300192 HeLa, Human Biozol Cat#ADD-C0008001 CHO-K1, hamster Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat#ACC110 ROMK2-Flp-In 293 Cells Renigunta et al.47 N/A Experimental models: Organisms/strains Strain NMY51: [MATa his3Δ200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ ade2::(lexAop)8-ADE2 GAL4] MoBiTec GmbH N/A Oligonucleotides mVSP pBT3C for: 5’-aat agg cca tta cgg cca tgt atg gag aa-3’ This paper N/A mVSP pBT3C rev: 5’-att tgg ccg agg cgg ccc tgt tct cac caa a-3’ This paper N/A mBasigin-pPR3C-for: 5’-att ggc cat tac ggc ccg atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin-pPR3C-rev: 5’-aat ggc cga ggc ggc cga ggt ggc gtt cct ctg-3’ This paper N/A mVSP-PEGFPC-for: 5’-gat ctc gag cta tgt atg gag aaa aga ag-3’ This paper N/A mVSP-PEGFPC-rev: 5’-ggt gga tcc tca ctg cag cta gtt ctc acc aaa-3’ This paper N/A mBasigin pRFPN for: 5’-gat ctc gag atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin pRFPN rev: 5’-ggt gga tcc cgg gtg gcg ttc ct-3’ This paper N/A mVSP exHA for: 5’-gca ctt tat gac aaa cca ggc ggg tac cca tac gac gtc cca gat tac gct ggc ggg cct cac ttt ctc aga g-3’ This paper N/A mVSP exHA rev: 5’-ctc tga gaa agt gag gcc cgc cag cgt aat ctg gga cgt cgt atg ggt acc cgc ctg gtt tgt cat aaa gtg c-3’ This paper N/A Recombinant DNA mVSP-pEGFPC This paper Genbank: NM_199257.2 mVSP-pRFP This paper Genbank: NM_199257.2 mVSP-pBT3N This paper Genbank: NM_199257.2 mVSP-3xflag-pCDNA3.1 This paper Genbank: NM_199257.2 mVSP-Ex-HA-pEGFP This paper Genbank: NM_199257.2 m/ci-VSP-pEGFP This paper N/A ci/m-VSP-pEGFP This paper N/A mBSG-pPR3C This paper Genbank: NM_001077184.1 mBSG-pRFP This paper Genbank: NM_001077184.1 mBSG-myc-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-3xflag-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-ERret-pRFP This paper N/A mEMB-pRFP This paper Genbank: NM_010330.4 mEMB -ERret-pRFP This paper N/A mNPTN-pRFP This paper Genbank: NM_009145.2 (Continued on next page) 18 Cell Reports 44, 116200, September 23, 2025
Enzyme-linked Immunosorbent Assay:Article Title: Electrochemical coupling at the plasma membrane by mouse voltage-sensitive phosphatase requires association with basigin.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFP Antibody (B-2) Santa Cruz Biotechnology Cat#sc-9996; RRID: AB_627695 ANTI-FLAG® M2 antibody Sigma-Aldrich Cat#F1804; RRID: AB_262044 Myc-Tag (71D10) Rabbit mAb Cell Signaling Technology Cat#91856; RRID: AB_3073821 Anti-Human HA Monoclonal Antibody Sigma-Aldrich Cat#H3663; RRID: AB_262051 Lex A (2-12) Santa Cruz Biotechnology Cat# sc-7544; RRID AB_627883 IRDye 800CW Goat anti-Mouse IgG LI-COR Biosciences Cat#925-32210; RRID: AB_2687825 IRDye 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 RDye 800CW Goat anti-Rat IgG LI-COR Biosciences Cat#926-32219, RRID: AB_1850025 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo Fisher Scientific Cat#A-11029; RRID: AB_2534088 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 594 Thermo Fisher Scientific Cat#A-11032; RRID: AB_2534091 Mouse EMMPRIN/CD147 Antibody R and D Systems Cat#AF772; RRID: AB_355588 Donkey anti-goat IgG-HRP Santa Cruz Biotechnology Cat# sc-2020; RRID: AB_631728 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Hygromycin B Thermo Fisher Scientific Cat#10687010 GelRed® Nucleic Acid Stain Merck Cat#SCT123 Protease-Inhibitor-Mix G SERVA Cat#39101 Triton® X 100 ROTH Cat#3051.3 Poly(ethyleneglycol) Sigma-Aldrich Cat#81242 Minimal SD Base Takara Cat#630411 DO Supplement –Leu/–Trp Takara Cat#630417 DO Supplement –His/–Leu/–Trp Takara Cat#630419 DO Supplement –Ade/–His/–Leu/–Trp Takara Cat#630428 YPD Broth ROTH Cat#X970 Lithium acetate dihydrate Sigma-Aldrich Cat#L4158 Deoxyribonucleic acid from herring sperm Sigma-Aldrich Cat#D3159 jetPEI® Polyplus Cat#101000053 jetPRIME® Polyplus Cat101000046 Critical commercial assays Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#M8823 Y2H Membrane Protein Kit MoBiTec GmbH Cat#P01001DS PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 Phusion High-Fidelity PCR Master Mix with HF Buffer Thermo ScientificTM Cat#F531L SuperSignalTM ELISA Femto Substrate Thermo ScientificTM Cat#37075 Deposited data Source data https://doi.org/10.6084/ m9.figshare.29665130 N/A (Continued on next page) Cell Reports 44, 116200, September 23, 2025 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines HEK-293, Human Cytion Cat#CLS-300192 HeLa, Human Biozol Cat#ADD-C0008001 CHO-K1, hamster Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Cat#ACC110 ROMK2-Flp-In 293 Cells Renigunta et al.47 N/A Experimental models: Organisms/strains Strain NMY51: [MATa his3Δ200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ ade2::(lexAop)8-ADE2 GAL4] MoBiTec GmbH N/A Oligonucleotides mVSP pBT3C for: 5’-aat agg cca tta cgg cca tgt atg gag aa-3’ This paper N/A mVSP pBT3C rev: 5’-att tgg ccg agg cgg ccc tgt tct cac caa a-3’ This paper N/A mBasigin-pPR3C-for: 5’-att ggc cat tac ggc ccg atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin-pPR3C-rev: 5’-aat ggc cga ggc ggc cga ggt ggc gtt cct ctg-3’ This paper N/A mVSP-PEGFPC-for: 5’-gat ctc gag cta tgt atg gag aaa aga ag-3’ This paper N/A mVSP-PEGFPC-rev: 5’-ggt gga tcc tca ctg cag cta gtt ctc acc aaa-3’ This paper N/A mBasigin pRFPN for: 5’-gat ctc gag atg gcg gcg gcg ctg ctg-3’ This paper N/A mBasigin pRFPN rev: 5’-ggt gga tcc cgg gtg gcg ttc ct-3’ This paper N/A mVSP exHA for: 5’-gca ctt tat gac aaa cca ggc ggg tac cca tac gac gtc cca gat tac gct ggc ggg cct cac ttt ctc aga g-3’ This paper N/A mVSP exHA rev: 5’-ctc tga gaa agt gag gcc cgc cag cgt aat ctg gga cgt cgt atg ggt acc cgc ctg gtt tgt cat aaa gtg c-3’ This paper N/A Recombinant DNA mVSP-pEGFPC This paper Genbank: NM_199257.2 mVSP-pRFP This paper Genbank: NM_199257.2 mVSP-pBT3N This paper Genbank: NM_199257.2 mVSP-3xflag-pCDNA3.1 This paper Genbank: NM_199257.2 mVSP-Ex-HA-pEGFP This paper Genbank: NM_199257.2 m/ci-VSP-pEGFP This paper N/A ci/m-VSP-pEGFP This paper N/A mBSG-pPR3C This paper Genbank: NM_001077184.1 mBSG-pRFP This paper Genbank: NM_001077184.1 mBSG-myc-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-3xflag-pCDNA3.1 This paper Genbank: NM_001077184.1 mBSG-ERret-pRFP This paper N/A mEMB-pRFP This paper Genbank: NM_010330.4 mEMB -ERret-pRFP This paper N/A mNPTN-pRFP This paper Genbank: NM_009145.2 (Continued on next page) 18 Cell Reports 44, 116200, September 23, 2025
Protease Inhibitor:Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat#87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2
Reverse Transcription:Article Title: TMEM145 is a principal component of outer hair cell stereocilia.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-TMEM145 polyclonal antibody Atlas Antibodies Cat# HPA060462; RRID:AB_2684285 TUB antibody Proteintech Cat# 17928-1-AP; RRID:AB_2212236 Anti-STRC polyclonal antibody Eurogentec N/A ARL13B antibody Proteintech Cat# 17711-1-AP; RRID:AB_2060867 Anti-KCNQ4 Antibody Alomone Labs Cat# APC-164; RRID:AB_2341042 Mouse Anti-Eps8 Monoclonal Antibody, Unconjugated, Clone 15 BD Biosciences Cat# 610144; RRID:AB_397545 Recombinant Anti-LHFPL5 antibody [EPR16285] - C-terminal Abcam Cat# ab192242; RRID:AB_2942040 Anti-CDH23 Antibody Prof. Ulrich Müller, John Hopkins University55 N/A DYKDDDDK tag antibody Proteintech Cat# 80801-2-RR; RRID: AB_3670488 V5-tag antibody Proteintech Cat# 81775-4-RR; RRID:AB_3670504 HA tag antibody Proteintech Cat# 51064-2-AP; RRID:AB_11042321 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 488 Conjugated Thermo Fisher Scientific Cat# A-11008; RRID:AB_143165 Goat Anti-Rabbit IgG (H+L) Antibody, Alexa Fluor® 568 Conjugated Thermo Fisher Scientific Cat# A-11011; RRID:AB_143157 Goat Anti-Mouse IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor® 647 Conjugated Thermo Fisher Scientific Cat# A-21236; RRID:AB_2535805 IRDye® 800CW Goat anti-Rabbit IgG LI-COR Biosciences Cat#926-32211; RRID: AB_621843 StarBright® Blue 700 Goat Anti-Mouse IgG Bio-Rad Cat# 12004159; RRID:AB_2884948 FluoTag®-X2 anti-ALFA AbberiorStar635P NanoTag Biotechnologies Cat# N1502-AB635P-L; RRID:AB_3075979 Bacterial and virus strains Subcloning EfficiencyTM DH5α Competent Cells Thermo Fisher Scientific Cat#18265017 Chemicals, peptides, and recombinant proteins Protease inhibitor cocktail Sigma-Aldrich Cat#P8349 PMSF solution Merck Cat#10837091001 Triton® X-100 ROTH Cat#3051.3 jetPRIME® Polyplus Cat101000046 Polyethylenimine (PEI) Custom-made N/A LipofectamineTM 2000 Transfection Reagent Thermo Fisher Scientific Cat#11668030 FM1-43 dye solution MedChemExpress Cat#HY-D1434 GSK-A1 SYNkinase Cat#SYN-1219-M001 DAPI Nucleic Acid Stain Thermo Fisher Scientific Cat#D1306 Phalloidin-iFluor 633 Reagent Abcam Cat#ab176758 Phalloidin-iFluor 405 Reagent Abcam Cat#ab176752 1,2-Bis(2-Aminophenoxy)ethaneN,N,N′,N′-tetraacetic acid (BAPTA) Sigma-Aldrich Cat#A4926 Poly-D-lysine Thermo Fisher Scientific Cat#A3890401 Critical commercial assays SuperScript IV Reverse Transcriptase Thermo Fisher Scientific Cat#18090010 (Continued on next page) ll OPEN ACCESS Article e1 Neuron 114, 1–15.e1–e7, August 5, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse brain total extracted RNA Ambion N/A Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530S PfuUltra II Hotstart PCR Master Mix Agilent Cat#600852 EZviewTM Red ANTI-FLAG® M2 Affinity Gel Millipore Cat#F2426 Experimental models: Cell lines HEK293, Human Thermo Fisher Scientific Cat#11625019 HeLa, Human Prof. Ralf Jakob, Marburg56 N/A LLC-PK1-CL4, Pig James R. Bartles57 N/A Experimental models: Organisms/strains C57BL/6NCrl Charles River N/A C57BL/6NCrlTmem145em1(IMPC)Mbp/Mmucd MMRRC RRID:MMRRC_065589-UCD Tmem145mandolin/mandolin Potter et al.26 N/A STRC-KO Shubina-Oleinik et al.15 N/A Oligonucleotides See Table S1 N/A Recombinant DNA mTmem145-pEGFP-N1 This paper NM_183311.2 mTmem145ΔC-pEGFP-N1 This paper NM_183311.2 mTmem145-HA-pCDNA3.1(+) Genescript NM_183311.2 mTmem145-alfatag-pCDNA3.1(-) Genescript NM_183311.2 mTmem145-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔGOLD-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mTmem145ΔC-3xFLAG-pCDNA3.1(+) This paper NM_183311.2 mSTRC-Myc-pCDNA3.1(+) This paper NM_021885.4; Subcloning from Shubina-Oleinik et al.15 TagBFP2-CAAX This paper N/A pcDNA3.1-MYO10-HMM-Nanotrap Bird et al.25 Addgene; Cat#87255 pEGFP-MYO10-HMM Bird et al.25 Addgene; Cat#87256 mTubby-DsREDmono-pEGFP-C1 Thallmair et al.42 NM_021885.4; mTubby-pRFP-C1 Thallmair et al.42 NM_021885.4; mTubby-V5-pCDNA3.1(+) Genescript NM_021885.4 Software and algorithms Graphpad Prism v5.0 GraphPad Software https://www.graphpad.com Igor Pro v8.0.4.2 Wavemetrics https://www.wavemetrics.com/ Fiji v2.14.0 Schneider et al.58 https://fiji.sc/ PYMOL v2.1.0 Schrodinger https://www.pymol.org/ Biorender Biorender https://www.biorender.com/ Adobe Illustrator v30.0 Adobe Inc. https://www.adobe.com/ Snapgene Dotmatics https://www.snapgene.com/ RStudio v4.4.0 Posit https://posit.co/ Superplot package Lord et al.59 N/A Interface Residues script SB Grid Consortium https://pymolwiki.org/InterfaceResidues NanoSPD ImageJ macro This paper DOI:10.5281/zenodo.18840150 Other AlphaFold Jumper et al.32 https://alphafoldserver.com ll OPEN ACCESSArticle Neuron 114, 1–15.e1–e7, August 5, 2026 e2
RNA Extraction:Article Title: Exonic splicing code and coordination of divalent metals in proteins.
Article Snippet: .. Transfections were carried out in 12- or 4-well plates using 150 ng of reporter DNA and jetPRIME Polyplus) according to the manufacturer’s recommendations. he cells were lysed 24 h later for RNA extraction. .. Total NA was isolated using TRI Reagent (Molecular Research enter) according to the manufacturer’s protocol and used or the first-strand cDNA synthesis with the Moloney murine eukaemia virus reverse transcriptase (Promega) and oligo (T) primers.
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